Aim of study: The effect of planting substrate and concentrations of indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) hormones was studied on the rooting of mugo pine cuttings.

Area of study: The research was carried out in Rasht city, Guilan province, Iran.

Material and Methods: Both hormones (IBA and NAA) were applied at four concentrations of 0, 1000, 2000 and 4000 mg/l. Planting substrates included sand, perlite, cocopeat, sand + perlite, and sand + cocopeat (1:1).

Main results: The highest rooting percentage (55%) was obtained under the trilateral treatment a₂b₄c₁ (sand × 4000 mg/l NAA × 1000 mg/l IBA). Sand + cocopeat was found to be the best rooting substrate.

Research highlights: It is recommended to apply sand with 4000 mg/l and 1000mg/l concentration of experimental hormones (NAA and IBA, respectively).

Keywords: auxin; rooting; Pinus mugo; vegetative propagation.